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Determination of free amino acids in microalgae by high‐performance liquid chromatography using pre‐column fluorescence derivatization
Author(s) -
Betancort Rodríguez J. R.,
García Reina G.,
Santana Rodríguez J. J.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199711)11:6<335::aid-bmc684>3.0.co;2-t
Subject(s) - chlorella pyrenoidosa , chemistry , chromatography , derivatization , alanine , amino acid , leucine , chlorella , fluorescence , high performance liquid chromatography , hydrolysate , arginine , isoleucine , hydrolysis , biochemistry , algae , ecology , physics , quantum mechanics , biology
An analytical method for a rapid reverse‐phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r>0.99) in the range 100–1000 nM. The proposed method was applied to the determination of free amino acids in two microalgae species ( Chlorella pyrenoidosa and Chlamydomonas rinhardtii ). The principal aminoacids found were alanine, arginine leucine, serine and glutamic acid. © 1997 John Wiley & Sons, Ltd.