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Direct Injection Analysis of Chlorzoxazone and its Major Metabolite 6‐Hydroxychlorzoxazone in Human Serum using a Semipermeable Surface (SPS) HPLC Column
Author(s) -
Haque A.,
Stewart J. T.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199707)11:4<236::aid-bmc679>3.0.co;2-i
Subject(s) - chromatography , chemistry , metabolite , chlorzoxazone , high performance liquid chromatography , acetonitrile , detection limit , reproducibility , in vitro , biochemistry , cyp2e1 , microsome
A semipermeable surface (SPS) HPLC column has been used to develop a direct injection method for the simultaneous determination of chlorzoxazone and its 6‐hydroxy metabolite in serum. Spiked serum was filtered and 20 μL injected directly onto the SPS column. The separation was achieved within 25 min using a mobile phase of 20:80 v/v acetonitrile–aqueous 0.05 m phosphate buffer pH 7 with detection at 280 nm. The method was linear in the concentration range 0.5–20 μg/mL ( r >0.999, n =6) with an RSD<5%. The limits of quantitation and detection were 0.5 μg/mL and 0.1 μg/mL (S/N>3), respectively, for both drug and metabolite. Intraday and interday reproducibility was ⩽6% with recoveries of drug and metabolite>94%. © 1997 John Wiley & Sons, Ltd.