Premium
Separation of Chiral Amino Acids by Micellular Electrokinetic Chromatography with Derivatized Cyclodextrins
Author(s) -
DeSilva Kapila,
Kuwana Theodore
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199707)11:4<230::aid-bmc678>3.0.co;2-2
Subject(s) - chemistry , micellar electrokinetic chromatography , chromatography , enantiomer , cyclodextrin , amino acid , elution , resolution (logic) , electrokinetic phenomena , analyte , chiral derivatizing agent , capillary electrophoresis , high performance liquid chromatography , organic chemistry , chiral column chromatography , biochemistry , artificial intelligence , computer science
The chiral separation of several amino acid (AA) enantiomers derivatized with naphthalene‐2,3‐dialdehyde (NDA) was achieved by use of cyclodextrin‐modified micellar electrokinetic chromatography (CD–MEKC). Both neutral hydroxypropyl β‐cyclodextrin (HP‐β‐CD) and charged carboxymethyl β‐cyclodextrin (CM‐β‐CD) were used as buffer additives for optical resolution of derivatized amino acids. The order of elution and the mechanism of separation for different CDs were explained by considering important chemical equilibria in the sodium dodecyl suphate, CD and amino acid system. Furthermore, the importance of SDS for the separation of a mixture of AAs, and the effect of CD and analyte concentration on the resolution will be discussed. © 1997 John Wiley & Sons, Ltd.