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Detection of Aequorea victoria Green Fluorescent Protein by Capillary Electrophoresis Laser Induced Fluorescence Detection
Author(s) -
Craig Douglas B.,
Wong Jerome C. Y.,
Dovichi Norman J.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199707)11:4<205::aid-bmc675>3.0.co;2-#
Subject(s) - capillary electrophoresis , aequorea victoria , chemistry , fluorescence , chromatography , laser induced fluorescence , green fluorescent protein , biochemistry , optics , gene , physics
Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post‐capillary laser‐induced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0×10 −12 m protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules. © 1997 John Wiley & Sons, Ltd.