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Insertion of MAP4‐VP1 Peptide Into Lipid Monolayers and Bilayers
Author(s) -
Mestres C.,
Haro I.,
Reig F.,
Alsina M. A.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199705)11:3<172::aid-bmc680>3.0.co;2-n
Subject(s) - chemistry , chromatography , peptide , monolayer , lipid bilayer , biophysics , membrane , biochemistry , biology
Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4‐VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role. © 1997 John Wiley & Sons, Ltd.