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Removal of Endotoxin from Aqueous Solutions by Affinity Membrane
Author(s) -
Guo Wei,
Shang Zhenhua,
Yu Yinian,
Zhou Liangmo
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199705)11:3<164::aid-bmc663>3.0.co;2-b
Subject(s) - chemistry , membrane , glutaraldehyde , hexamethylenediamine , chromatography , aqueous solution , histidine , ligand (biochemistry) , affinity chromatography , matrix (chemical analysis) , polymer chemistry , organic chemistry , biochemistry , polyamide , amino acid , receptor , enzyme
Endotoxin was removed by affinity membranes with histidine immobilized as affinity ligand. Macropore cellulose membrane was prepared from filter paper by alkaline treatment and chemical crosslinking, and was used as matrix for the immobilization of affinity ligand. The matrix membrane was derived by hexamethylenediamine and activated by glutaraldehyde before histidine was immobilized. Membrane cartridges containing 40 or 80 sheets of affinity membrane were also prepared, which can be used to remove pyrogen from aqueous solutions. Using a cartridge with 40 sheets of affinity membrane, the endotoxin content in solution can be reduced to a minimum of 0.12 EU/mL. © 1997 John Wiley & Sons, Ltd.