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Systematic Analysis of Post‐Administrative Saiboku‐To Urine by Liquid Chromatography to Determine Pharmacokinetics of Traditional Chinese Medicine
Author(s) -
Homma Masato,
Oka Kitaro,
Taniguchi Chizu,
Niitsuma Tomoyuki,
Hayashi Tohru
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199705)11:3<125::aid-bmc631>3.0.co;2-l
Subject(s) - chemistry , magnolol , chromatography , high performance liquid chromatography , wogonin , urine , scutellaria baicalensis , traditional chinese medicine , medicine , biochemistry , alternative medicine , pathology
To disclose the mystery of a traditional Chinese medicine and to identify biologically active components, we analysed post‐administrative urine for Saiboku‐To, an anti‐asthmatic Chinese herbal remedy. Systematic analysis of the components appearing in the urine was carried out by high‐performance liquid chromatography (HPLC) with normal‐ and reversed‐phase modes in combination. β‐ D ‐glucuronidase‐treated urine was subjected to rapid‐flow fractionation (RFF) to achieve fractional extraction of lipophilic components with exhaustive recovery rates. The extracts were analysed by HPLC equipped with a multi‐channel UV‐detector. In the first stage of HPLC, we conducted a normal‐phase mode run to find magnolol derived from Magnolia officinalis , as the most hydrophobic component showing minimum retention time among the urinary products of Saiboku‐To. In the next stage, mobile phase solvent composition for reversed‐phase HPLC was optimized so as to retain magnolol up to 60 min. Under these conditions, other Saiboku‐To urinary products, which were more polar than magnolol, appeared within 60 min. Our HPLC method used marker compounds like magnolol and could indicate the terminal peak position on the reversed‐phase chromatography. We found a total of eight components in the post‐administrative Saiboku‐To urine. Structure identification of the isolated pure materials was achieved using nuclear magnetic resonance (NMR)‐, mass (MS)‐ and UV‐spectra, and HPLC retention profiles. They were magnolol and 8,9‐dihydroxydihydromagnolol stemming from M. officinalis , medicarpin and liquiritigenin from Glycyrrhiza glabra , baicalein, wogonin, and oroxylin A from Scutellaria baicalensis , and davidigenin of an unknown origin. The pharmacological mystery of Saiboku‐To should be disclosed by resolving the pharmacokinetics and pharmacodynamics of these urinary products independently and synergistically. © 1997 John Wiley & Sons, Ltd.