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Direct Assay of Tinidazole in Human Serum by Micellar Liquid Chromatography
Author(s) -
Habel D.,
Guermouche S.,
Guermouche M. H.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199701)11:1<16::aid-bmc619>3.0.co;2-k
Subject(s) - tinidazole , chromatography , chemistry , micellar liquid chromatography , high performance liquid chromatography , detection limit , extraction (chemistry) , reproducibility , pharmacokinetics , solvent , micelle , pharmacology , biochemistry , antibiotics , aqueous solution , medicine , metronidazole
A liquid chromatographic procedure for the direct determination of tinidazole in human serum is presented. It includes the use of a micellar mobile phase consisting of SDS (5.10 −2 M ): propan‐1‐ol; (94:6; v/v) and a μBondapak CN column with UV detection at 320 nm. No solvent extraction or deproteinization are necessary. The linearity (0.1–10 mg L), the precision (3%), the reproducibility (1.3%), the recovery (99%), and the detection limit (0.1 mg L) in the tinidazole determination are comparable and sometimes greater than the corresponding tinidazole parameters when deproteinization and conventional reversed‐phase HPLC are used. One hundred injections of serum samples do not affect the column life. The procedure is applied to ascertain the pharmacokinetics of 10 mg/kg of tinidazole. © 1997 by John Wiley & Sons, Ltd.