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Systematic Multivariate Optimization of Separation of Auxins by Capillary Electrophoresis Based on Overlapping Resolution Mapping Scheme
Author(s) -
Liu J.,
Li S. F. Y.
Publication year - 1997
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199701)11:1<1::aid-bmc606>3.0.co;2-#
Subject(s) - capillary electrophoresis , resolution (logic) , chemistry , chromatography , separation (statistics) , scheme (mathematics) , multivariate statistics , capillary action , electrophoresis , buffer (optical fiber) , high resolution , analytical chemistry (journal) , biological system , computer science , artificial intelligence , mathematics , machine learning , materials science , mathematical analysis , remote sensing , composite material , biology , geology , telecommunications
In this paper, the overlapping resolution mapping (ORM) scheme was applied in the separation of 15 plant auxins by capillary electrophoresis. An interpretative optimization approach was utilised to predict the optimum buffer pH, and the concentrations of α‐, β‐, and γ‐cyclodextrins. A four‐dimensional overlapping resolution mapping scheme was developed to optimize these four parameters simultaneously. By conducting sixteen prelanned experiments, global optimum conditions for the separation within an acceptable analysis time could be obtained. © 1997 by John Wiley & Sons, Ltd.