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HPLC of Sertraline and Norsertraline in Plasma or Serum
Author(s) -
Patel Jignasha,
Spencer E. P.,
Flanagan R. J.
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199611)10:6<351::aid-bmc620>3.0.co;2-o
Subject(s) - chemistry , chromatography , detection limit , analyte , high performance liquid chromatography , elution , calibration curve , methanol , aqueous solution , organic chemistry
A simple method for the measurement of sertraline and norsertraline in plasma or serum suitable for use in single‐dose pharmacokinetic studies has been developed. Internal standard solution, aqueous fenethazine (10 mg/L) (20 μL), and Tris buffer (2 mol/L), pH 10.6) (100 μL) were added to plasma (200 μL). Sertraline, norsertraline and the internal standard were extracted into methyl tert ‐butyl ether (200 μL) by mixing (30 s) and centrifugation (11,000 r.p.m., 4 min). A portion (100 μL) of the extract was injected onto a Spherisorb S5SCX HPLC column (150×4.6 mm i.d.) which was eluted with methanol:water (19+1) containing ammonium perchlorate (40 mmol/L), final pH 7.0. Detection was by UV monitoring (215 nm). The concentration of each analyte in each sample was calculated from the calibration graph (peak‐height ratio of analyte to that of the internal standard against analyte concentration) obtained after analysis of plasma samples containing known amounts of sertraline and norsertraline. The limit of accurate measurement of the assay was 10 μg/L) sertraline and 20 μg/L)norsertraline.