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Measurement of Bioactive Neuropeptides Using a Chromatographic Immunosensor Cartridge
Author(s) -
Phillips Terry M.
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199611)10:6<331::aid-bmc614>3.0.co;2-f
Subject(s) - neuropeptide , chemistry , analyte , calcitonin , chromatography , peptide , vasoactive intestinal peptide , bioassay , calcitonin gene related peptide , receptor , cartridge , biochemistry , medicine , biology , mechanical engineering , engineering , genetics
Neuropeptide modulation of host defenses is associated with a number of clinical conditions in which tissue injury is caused by a process termed neurogenic inflammation. Monitoring of neuropeptide activity during both disease episodes and therapy has proved difficult due to a lack of correlation between laboratory evaluations and disease activity. Although a number of assays are available for measuring the total neuropeptide content of tissue samples, few can measure the bioactive component which is the more physiologically accurate parameter. In an attempt to overcome this problem, a receptor‐affinity chromatographic technique coupled with immunological detection has been developed for measuring three bioactive neuropeptides (substance P, vasoactive intestinal peptide, and calcitonin gene‐related peptide) in tissue biopsy extracts. The technique involves isolation of the active neuropeptides via their ability to bind to immobilized receptors followed by measurement of the bound materials by a series of simultaneous immunoassays. The technique compares favourably with results obtained using conventional bioassays and has the added advantage that multiple analytes can be measured in a single procedure.