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Carbohydrate Chromatography: Towards Yoctomole Sensitivity
Author(s) -
Davies M. J.,
Hounsell E. F.
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199611)10:6<285::aid-bmc616>3.0.co;2-w
Subject(s) - chemistry , chromatography , capillary electrophoresis , chromogenic , oligosaccharide , fluorescence , high performance liquid chromatography , resolution (logic) , biochemistry , optics , physics , artificial intelligence , computer science
In attempting to analyse complex carbohydrates by HPLC or capillary electrophoresis (CE) the chromatographer is faced with many challenges. The diversity of oligosaccharide structures described to date and their poor resolution on many chromatographic systems present a significant challenge to the chromatographer. In addition to this, the non‐chromogenic nature of most carbohydrates greatly decreases the sensitivity of UV detection and refractive index is not an option for sensitive analysis. Over the last five years there have been significant advances in separation and detection methods for carbohydrates, and in this paper we will attempt to describe the most significant advances and highlight potential future developments. The detection of 200–300 fmol (2–3×10 −13 mol) of oligosaccharides can now be readily achieved using electrochemical detection or fluorescence detection (after labelling) with conventional HPLC columns and detectors. Miniaturized separation systems have greatly increased sensitivities with a CE‐laser induced fluorescence instrument detecting as little as 100 yoctomoles (1×10 −22 mol).