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Urinary Porphyrins and Heme Biosynthetic Enzyme Activities Measured byHPLC in Arsenic Toxicity
Author(s) -
GarcíaVargas Gonzalo G.,
HernándezZavala Araceli
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199611)10:6<278::aid-bmc613>3.0.co;2-x
Subject(s) - uroporphyrinogen iii decarboxylase , chemistry , porphobilinogen deaminase , protoporphyrinogen oxidase , ferrochelatase , heme , biochemistry , enzyme , porphyria , porphobilinogen , toxicity , arsenic , biology , organic chemistry , endocrinology
Arsenic can modify the urinary excretion of porphyrins in animals and humans. Arsenic also interferes with the activities of several enzymes of the heme biosynthesis pathway, such as aminolevulinate synthase (ALA‐S), porphobilinogen deaminase (PBGD), uroporphyrinogen III synthase (Uro III S), uroporphyrinogen decarboxy‐ lase (URO‐D), coproporphyrinogen oxidase (COPRO‐O), ferrochelatase and heme oxygenase (H‐O). This review deals with HPLC‐based techniques for the analysis of porphyrins in human and rodent urine and several heme enzymes with discussion on their usefulness as early biomarkers of arsenic toxicity.