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Thin‐layer Chromatographic Determination of Catecholamines in Rat Plasma
Author(s) -
Alemany G.,
Akaârir M.,
Rosselló C.,
Gamundi A.
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199609)10:5<225::aid-bmc587>3.0.co;2-t
Subject(s) - chemistry , chromatography , densitometry , epinephrine , thin layer chromatography , norepinephrine , dopamine , isoprenaline , endocrinology , medicine , physics , quantum mechanics , stimulation
A TLC method for the quantitative determination of epinephrine, norepinephrine and dopamine in rat plasma is described. After deprotenization, catecholamines were adsorbed on acid–alumina and acetylated. The acetyl derivatives were extracted using a C18 minicolumn, resolved on HPTLC plates and quantitated by fluorescence densitometry at 415 nm, using isoprenaline as internal standard.