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Ultraviolet and Fluorescence Derivatization Reagents for Carboxylic Acids Suitable for High Performance Liquid Chromatography: A Review
Author(s) -
Mukherjee Partha S.,
Karnes H. Thomas
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199609)10:5<193::aid-bmc591>3.0.co;2-b
Subject(s) - derivatization , chemistry , reagent , chromatography , fluorophore , analyte , fluorescence , chromophore , high performance liquid chromatography , alkyl , carboxylic acid , organic chemistry , physics , quantum mechanics
Pre‐ and postcolumn derivatization with a suitable chromophore or fluorophore have often been utilized to enhance the sensitivity and selectivity of detection for analytes possessing a carboxyl group. The major classes of UV and fluorescent derivatization reagents include the coumarin analogues, alkyl halides, diazoalkanes, and amines. The derivatization reaction conditions, HPLC systems for separation of the conjugates and detection limits of various analytes are presented in this review. High mass sensitivity for various carboxylic acids have been achieved with the hydrazides, anthracenes, and diazoalkanes.