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Sensitive Detection of Near‐Infrared Fluorescent Dyes Using High‐Performance Liquid Chromatography with Peroxyoxalate Chemiluminescence Detection System
Author(s) -
Kimoto Kohsuke,
Gohda Ryoya,
Murayama Katsuhisa,
Santa Tomofumi,
Fukushima Takeshi,
Homma Hiroshi,
Imai Kazuhiro
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199607)10:4<189::aid-bmc585>3.0.co;2-p
Subject(s) - peroxyoxalate , chemistry , chemiluminescence , chromatography , fluorescence , detection limit , oxalate , high performance liquid chromatography , methylene blue , hydrogen peroxide , iodide , pyridine , photochemistry , inorganic chemistry , organic chemistry , catalysis , physics , quantum mechanics , photocatalysis
The highly sensitive detection of four near‐infrared (near‐IR) fluorescent dyes, methylene blue, pyridine 1, oxazine 1 and 3,3′‐diethylthiadicarbocyanine iodide (DTDCI) by high‐performance liquid chromatography (HPLC) with post‐column peroxyoxalate chemiluminescence (PO‐CL) detection utilizing bis(4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl) oxalate (TDPO) and hydrogen peroxide was examined. The detection limits for methylene blue, pyridine 1, oxazine 1 and DTDCI were 120, 27, 31, 0.19 fmol on‐column at a signal‐to‐noise ratio of 2, respectively. The sensitivity for DTDCI was 250 times that obtained by HPLC with the conventional fluorescent detection.