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High‐Performance Liquid Chromatography of α‐Amino acids and Aztreonam on Reversed Phase Columns with Aqueous Cu 2+ as Eluent
Author(s) -
Khedr Alaa
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199607)10:4<167::aid-bmc584>3.0.co;2-#
Subject(s) - chemistry , chromatography , aqueous solution , aztreonam , high performance liquid chromatography , elution , column chromatography , amino acid , methanol , phase (matter) , organic chemistry , imipenem , antibiotics , biochemistry , antibiotic resistance
Parameters affecting the separation of amino acids on different RP‐HPLC columns were studied. Six amino acids were separated on Zorbax TMS, Zorbax CN, Zorbax ODS and Zorbax C 8 , using 1.8×10 −3 M copper sulphate at pH 4.1 as aqueous mobile phase. The best separation was shown by Zorbax TMS followed by Zorbax CN. The column performance was maintained by serial washing after 6 h continuous work with aqueous 10 −4 M disodium edetate, 10 −4 N sulphuric acid, water and gradient elution with aqueous methanol. The separation mechanism was interpreted. The method was applied for separation and quantification of aztreonam and L ‐arginine in Azactam vials.