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Quantification of 9‐Carboxy‐11‐nor‐Δ 9 ‐tetrahydrocannabinol in Urine Using Brominated 9‐Carboxy‐11‐nor‐Δ 9 ‐tetrahydrocannabinol as the Internal Standard and High‐Performance Liquid Chromatography with Electrochemical Detection
Author(s) -
Fisher Daniel H.,
Broudy Marc I.,
Fisher L. Megan
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199607)10:4<161::aid-bmc579>3.0.co;2-3
Subject(s) - chromatography , chemistry , urine , tetrahydrocannabinol , detection limit , mass spectrometry , linear range , extraction (chemistry) , coefficient of variation , immunoassay , linear regression , cannabinoid , antibody , immunology , biochemistry , receptor , machine learning , computer science , biology
A method was developed for quantitating 9‐carboxy‐11‐nor‐Δ 9 ‐tetrahydrocannabinol in human urine as part of the process for validating an automated enzyme immunoassay for marijuana metabolites. Sample cleanup was accomplished using a mixed‐mode solid‐phase extraction. 9‐Carboxy‐11‐nor‐Δ 9 ‐tetrahydrocannabinol and the internal standard, brominated 9‐carboxy‐11‐nor‐Δ 9 ‐tetrahydrocannabinol, were quantified using high‐performance liquid chromatography with electrochemical detection (+0.85 V). The linear range for this method is 0.012–0.20 μg/mL. No interference was seen for 22 drugs and metabolites. The pooled relative standard deviation is 4.1% ( n =27) for the quality control samples. This method was compared to gas chromatography with mass spectrometry by linear regression analysis. The slope of the line is 1.00±0.05 (standard error), the intercept is approximately zero, the coefficient of determination is 0.994, and the standard error of the estimate is 0.006 μg/mL.