Premium
Simultaneous Determination of Vanillylmandelic, Homovanillic and 5‐Hydroxyindoleacetic Acids in Human Urine by Thin Layer Chromatography
Author(s) -
Alemany G.,
Gamundí A.,
Rosselló C.,
Rial R.
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199605)10:3<144::aid-bmc570>3.0.co;2-s
Subject(s) - chemistry , vanillylmandelic acid , chromatography , acetic acid , homovanillic acid , reagent , urine , densitometry , thin layer chromatography , 5 hydroxyindoleacetic acid , organic chemistry , biochemistry , physics , receptor , quantum mechanics , serotonin
A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5‐hydroxyindole‐3‐acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high‐performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with β‐(4‐hydroxy‐3‐phenyl) acetic acid as the internal standard.