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Rapid and Specific Precolumn Extraction High‐Performance Liquid Chromatographic Assay for Bupivacaine in Human Serum
Author(s) -
Emara Samy,
Khedr Alaa,
Askal Hassan
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199605)10:3<131::aid-bmc572>3.0.co;2-w
Subject(s) - chromatography , chemistry , extraction (chemistry) , high performance liquid chromatography , bupivacaine , quantitative analysis (chemistry) , sample preparation , pharmacology , medicine
A rapid and specific HPLC assay for quantitative determination of bupivacaine in human serum is described. The technique incorporates an on‐line sample clean‐up system followed by reversed‐phase chromatography with UV detection. The proposed method uses a column‐switching technique and protein‐coated Lichrosorb RP‐8 as a precolumn together with Lichrosorb RP‐18 as an analytical column. The total run time for an injection of serum sample was 10 min. This procedure offers a sensitive assay without the need for time‐consuming extractions. The average bupivacaine recoveries over a concentration range of 150–600 ng/mL ranged from 99.12 to 101.02%, and relative standard deviations ranged from 1.15 to 1.78%.