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Determination of Hydrophobicity Parameters of Antibiotics by Reversed‐Phase Chromatography. The Effect of Support
Author(s) -
Cserháti Tibor,
Oros Gyula
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199605)10:3<117::aid-bmc576>3.0.co;2-4
Subject(s) - chemistry , chromatography , phase (matter) , homologous series , methanol , antibiotics , stationary phase , silica gel , hydrophilic interaction chromatography , high performance liquid chromatography , organic chemistry , biochemistry
The hydrophobicity and specific hydrophilic surface area of 29 antibiotics were determined by reversed‐phase thin‐layer chromatography using impregnated silica and alumina supports and methanol:water mixtures as eluents. It was found that the supports retain their original retention characteristics even after impregnation influencing the reversed‐phase retention of antibiotics. It was established that the hydrophobicity parameters are intercorrelated both on impregnated silica and alumina layers, indicating that antibiotics behave as a homologous series of solutes. However, the correlations were not strong enough to substitute the parameters with each other in quantitative structure–activity relationship studies. The specific hydrophobic surface areas determined on impregnated silica and alumina showed no significant correlation, proving the decisive role of support characteristics in the determination of this parameter.