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Temperature Dependence of Retention in Reversed‐Phase Liquid Chromatography on a Porous Acrylic Support
Author(s) -
Kamiyama Fumio,
Yamazaki Kazutoshi,
Kawamura Kenichi,
Kohara Minoru
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199605)10:3<105::aid-bmc564>3.0.co;2-i
Subject(s) - chemistry , adsorption , polyethylene glycol , chromatography , copolymer , polyethylene , porosity , phase (matter) , stationary phase , hydrophilic interaction chromatography , column chromatography , reversed phase chromatography , chemical engineering , high performance liquid chromatography , organic chemistry , polymer , engineering
The solute retention mechanism of reversed‐phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols increases with temperature. Adsorption behaviour of polyethylene glycol onto the copolymer beads shows that the adsorption also increases with temperature. These observations are explained on the basis of the hypothesis that hydrophobic interaction plays a vital role in solute retention.