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High‐Performance Liquid Chromatography of Long Chain Aliphatic Aldehydes with Peroxyoxalate Chemiluminescence Detection Utilizing a Fluorogenic Reagent, 4‐( N , N ‐Dimethylaminosulphonyl)‐7‐hydrazino‐2,1,3‐benzoxadiazole
Author(s) -
Nakashima Kenichiro,
Yoshida Tomomi,
Kuroda Naotaka,
Akiyama Shuzo
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199603)10:2<99::aid-bmc553>3.0.co;2-h
Subject(s) - chemistry , peroxyoxalate , chemiluminescence , chromatography , reagent , high performance liquid chromatography , organic chemistry
A peroxyoxalate chemiluminescence detection in high‐performance liquid chromatography was examined for determination of long chain aliphatic aldehydes using 4‐( N , N ‐dimethylaminosulphonyl)‐7‐hydrazino‐2,1,3‐benzoxadiazole (DBD‐H) as a fluorogenic labelling reagent. A mixture of 0.6 m M bis(2,4,6‐trichlorophenyl)oxalate and 100 m M H 2 O 2 in acetonitrile was used as a post‐column chemiluminescent reagent. DBD‐hydrazones of five long chain aliphatic aldehydes (C 11 –C 18:1 ) were eluted and well separated within 16 min with a mobile phase of 5 m M imidazole in acetonitrile. The detection limits were 14–18 fmol at a signal‐to‐noise ratio (S/N) of 2. DBD‐hydrazones of four aliphatic aldehydes (C 6 –C 11 ) were also eluted and well separated within 30 min with a mobile phase of 5 m M imidazole buffer (pH 7.5)‐acetonitrile (20/80, v/v). The detection limits were 86–152 fmol (S/N=2).