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Analysis of thiol‐containing compounds in biological samples by capillary zone electrophoresis
Author(s) -
Ercal Nuran,
Le Kang,
Treeratphan Piyanee,
Matthews Richard
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199601)10:1<15::aid-bmc544>3.0.co;2-5
Subject(s) - chemistry , capillary electrophoresis , derivatization , chromatography , capillary action , maleimide , phosphate buffered saline , electrophoresis , thiol , buffer (optical fiber) , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , telecommunications , materials science , computer science , composite material
A new method for the determination of reduced and oxidized glutathione by using capillary zone electrophoresis was developed and compared with the N ‐(1‐pyrenyl)maleimide high‐performance liquid chromatography method. The tissue samples were obtained from C57BL/6 mice and homogenized in deionized water. The detection wavelength was set at 214 nm. A 50‐cm effective length × 75 μm i.d. uncoated fused silica capillary with a 10 mM phosphate buffer at pH 7.5 was used for the experiment. Samples were introduced by pressure injection for 5 s. The voltage for separation was at 20 kV. Between runs, the capillary was regularly washed with 0.1 N NaOH and deionized water, followed by reconditioning with running buffer. The present method was shown to be reproducible and is convenient in that a derivatization step is not required. The method has been used to measure glutathione levels in biological samples and the results show good agreement with those obtained by high‐performance liquid chromotography.