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Use of silica gel with reversed‐phase eluents for the separation and determination of alkaloids in Corydalis Yanhusuo W. T. Wang and its preparations
Author(s) -
YongFang Yuan,
ZhengLiang Liu,
XiuLu Li
Publication year - 1996
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/(sici)1099-0801(199601)10:1<11::aid-bmc541>3.0.co;2-q
Subject(s) - chemistry , tetrahydropalmatine , chromatography , corydalis , high performance liquid chromatography , silica gel , phosphate buffered saline , phase (matter) , methanol , organic chemistry , medicine , alternative medicine , pathology , traditional chinese medicine
An HPLC method using unmodified silica with reversed‐phase eluents was developed for the determination of DL ‐tetrahydropalmatine in Corydalis Yanhusuo W. T. Wang and its set prescription preparations. The mobile phase was a mixture of methanol and 2 mmol/L phosphate buffer (75:25, v/v). The result was calculated by external standardization. The method is simple, rapid and precise, and could also be used for the separation and determination of alkaloids in other crude drugs and preparations.