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Inorganic and organic selenium compound speciation with coupled HPLC–MW–HG–AFS
Author(s) -
GómezAriza J. L.,
SánchezRodas D.,
Morales E.,
Herrgott O.,
Marr I. L.
Publication year - 1999
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199910)13:10<783::aid-aoc907>3.0.co;2-7
Subject(s) - chemistry , selenate , selenium , selenocysteine , detection limit , high performance liquid chromatography , hydride , chromatography , aqueous solution , genetic algorithm , seawater , inorganic chemistry , metal , organic chemistry , cysteine , evolutionary biology , biology , enzyme , oceanography , geology
Coupling between high‐performance liquid chromatography, microwave‐induced reduction, hydride generation and atomic fluorescence spectrometry (HPLC–MW–HG–AFS) has been used for selenium speciation. The concentrations of a redox mixture (HCl solution of KBr and KBrO 3 aqueous solutions) used for both destruction of organic matter and reduction of the selenium species have been optimized, as well as the concentration of NaBH 4 in the hydride generation step. Chromatographic separation of selenocysteine, selenite and selenate took place in 15 min, employing a polymer‐based strong anion‐exchange column. Calibrations with correlation coefficients r > 0.998 were obtained for the three compounds at concentrations between 0.5 and 10 µg l −1 . Detection limits for selenite, selenocysteine (SeCys) and selenate were 0.2, 0.3 and 0.5 µg l −1 , respectively. The coupling has been tested with spiked water samples (tapwater and seawater), obtaining recoveries in the range 94–104%. Copyright © 1999 John Wiley & Sons, Ltd.