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Organometallic complexes with biological molecules: XIII. Organotin(IV)[ meso ‐tetra (4‐carboxyphenyl)porphinate]s and the cell cycle: a flow‐cytometric approach
Author(s) -
Triolo F.,
Pellerito C.,
Stocco G. C.,
Fiore T.,
Maggio F.,
Pellerito L.,
Triolo R.
Publication year - 1999
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199910)13:10<733::aid-aoc923>3.0.co;2-m
Subject(s) - chemistry , tetra , flow cytometry , cell cycle , tributyltin , cytotoxicity , stereochemistry , cytotoxic t cell , in vivo , cell , in vitro , biochemistry , medicinal chemistry , microbiology and biotechnology , organic chemistry , biology
The cytotoxic derivatives diorganotin(IV) and triorganotin(IV) [ meso ‐tetra(4‐carboxyphenyl)porphinates, with stoichiometries [R 2 Sn] 2 TPPC and [R 3 Sn] 4 TPPC [R = Me, Bu, Ph; TPPC 4− = meso ‐tetra(4‐carboxyphenyl)porphinate 4− ], namely bis[dimethyltin(IV)], bis[dibutyltin(IV)], bis[diphenyltin(IV)], tetra[trimethyltin(IV)], tetra[tributyltin(IV)] and tetra[triphenyltin(IV)] [ meso ‐tetra(4‐carboxyphenyl)porphinate]s, have been used to investigate their effects on the cultured human kidney cell cycle in order to understand further the origin of cell‐growth inhibition induced by the above‐mentioned chemicals. The cell‐cycle‐dependent DNA content distribution of cultured cells exposed to these compounds has been analyzed through flow cytometry, a potent technique capable of probing several aspects of drug‐induced cytotoxicity. Cultured human kidney cells have been used as a model system, on the premise of greater physiological similarity to the human situation in vivo . Copyright © 1999 John Wiley & Sons, Ltd.