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Redox labeling of two antiepileptic drugs with metallocenes and their simultaneous detection by a Nafion‐modified electrode
Author(s) -
Bordes AnneLine,
Schöllhorn Bernd,
Limoges Benoît,
Degrand Chantal
Publication year - 1998
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199801)12:1<59::aid-aoc672>3.0.co;2-y
Subject(s) - chemistry , nafion , phenobarbital , phenytoin , redox , voltammetry , derivative (finance) , detection limit , electrode , cationic polymerization , nuclear chemistry , inorganic chemistry , chromatography , electrochemistry , organic chemistry , epilepsy , medicine , neuroscience , financial economics , economics , pharmacology , biology
Two different cationic redox labels, i.e. a ferroceneammonium ion and a cobaltocenium ion, were covalently attached to two antiepileptics, phenytoin and phenobarbital, respectively. The two labeled drugs possess distinct standard redox potentials of 0.39 V for the phenytoin derivative and −0.92 V for phenobarbital derivative (vs Ag/AgCl, Cl − 0.05 M ) at a carbon paste electrode. After preconcentration in a polyanionic Nafion‐loaded carbon paste electrode the positively charged labeled phenytoin and phenobarbital derivatives could be simultaneously detected in concentration ranges which were relevant to the therapeutic ranges of the antiepileptics, with a view to a future dual‐analyte immuno‐ assay. Square‐wave voltammetry permitted detection limits of 5×10 −8 M (for the phenytoin derivative) and 2.5×10 −8 M (for the phenobarbital derivative) for non‐simultaneous detection. © 1998 John Wiley & Sons, Ltd.