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Arsenic Compounds in Terrestrial Organisms II: Arsenocholine in the Mushroom Amanita muscaria
Author(s) -
Kuehnelt Doris,
Goessler Walter,
Irgolic Kurt J.
Publication year - 1997
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199706)11:6<459::aid-aoc583>3.0.co;2-o
Subject(s) - arsenobetaine , chemistry , arsenate , arsenic , arsenite , bromide , chromatography , environmental chemistry , nuclear chemistry , inorganic chemistry , organic chemistry
Arsenic compounds were identified and quantified in the mushroom Amanita muscaria, collected close to a facility that had roasted arsenic ores. The powdered dried mushrooms were extracted with methanol/water (9:1), the extracts were concentrated and the concentrates were dissolved in water. The resulting solutions were chromatographed on anion‐exchange, cation‐exchange and reversed‐ phase columns. Arsenic was detected on‐line with an ICP–MS detector equipped with a hydraulic high‐pressure nebulizer. Arsenite, arsenate, dimethylarsinic acid and the tetramethylarsonium cation were minor arsenic compounds (∼2% each of the total 22 mg kg −1 dry mass), and arsenobetaine, arsenocholine (∼15% each) and several unidentified arsenic compounds (∼60%) were the major arsenic compounds in Amanita muscaria. The presence of arsenocholine (detected for the first time in a terrestrial sample) was ascertained by matching retention times in the anion‐exchange, cation‐ exchange and reversed‐phase chromatograms with the retention time of synthetic arsenocholine bromide and chromatographing extracts spiked with arsenocholine bromide. © 1997 John Wiley & Sons, Ltd.