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GC–SIM/MS Profiling of Urinary Steroids as their Per‐trimethylsilyl Derivatives
Author(s) -
BakolaChristianopoulou M. N.,
Apazidou K. K.,
Psarros L.
Publication year - 1997
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199703)11:3<205::aid-aoc542>3.0.co;2-t
Subject(s) - chemistry , trimethylsilyl , derivatization , steroid , chromatography , gas chromatography–mass spectrometry , gas chromatography , solvent , iodide , organic chemistry , bstfa , methyl iodide , urine , mass spectrometry , biochemistry , hormone
Complete GC–SIM/MS separation and identification of 19 steroids (including several steroid isomers) in a male urine sample, as their per‐trimethylsilyl ethers, is described. Trimethylsilylation of their functional groups (hydroxyl and carbonyl) was achieved by a rapid, simplified and one‐step derivatization method, using N ‐methyl‐ N ‐trimethylsilyl‐2,2,2,‐trifluoroacetamide as the silylating agent and the solvent as well, along with catalytic amounts of trimethylsilyl iodide and dithioerythritol. Furthermore, a GC/MS method for the analysis of a synthetic mixture of underivatized urinary steroids is proposed. © 1997 by John Wiley & Sons, Ltd.