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Arsenic Compounds Accumulated in Sedimentary Microorganisms Cultivated in Media Containing Several Arsenicals
Author(s) -
Hanaoka Ken'ichi,
Dote Yosio,
Yosida Kenta,
Kaise Toshikazu,
Kuroiwa Takayosi,
Maeda Sigeru
Publication year - 1996
Publication title -
applied organometallic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 71
eISSN - 1099-0739
pISSN - 0268-2605
DOI - 10.1002/(sici)1099-0739(199611)10:9<683::aid-aoc532>3.0.co;2-1
Subject(s) - arsenobetaine , chemistry , arsenic , microorganism , environmental chemistry , chromatography , salt (chemistry) , inorganic arsenic , organic chemistry , bacteria , biology , genetics
Sediments, as sources of microorganisms, were added to two kinds of media, 1/5 ZoBell 2216E and a solution of inorganic salts, which contained inorganic arsenic(III), inorganic arsenic(V), methanearsonic acid, dimethyl‐ arsinic acid, trimethylarsine oxide, tetramethylarsonium salt or arsenocholine. After 17 days of incubation at 20 °C, the arsenicals that had accumulated in the microorganisms were analysed by high‐performance liquid chromatography (HPLC). While the more toxic arsenicals [inorganic arsenic(III), inorganic arsenic(V), methanearsonic acid, dimethylarsinic acid] were not converted in the microorganisms, trimethylarsine oxide and tetramethylarsonium salt were considerably degraded to inorganic arsenic(V), and arsenocholine to arsenobetaine. Arsenobetaine that had accumulated in the microorganisms was extracted and confirmed by thin‐layer chromatography (TLC) and fast atom bombardment (FAB) mass spectrometry.