Premium
Synthesis of Pyridinium Amphiphiles Used for Transfection and Some Characteristics of Amphiphile/DNA Complex Formation
Author(s) -
Meekel Arthur A. P.,
Wagenaar Anno,
Šmisterová Jarmila,
Kroeze Jessica E.,
Haadsma Peter,
Bosgraaf Bouke,
Stuart Marc C. A.,
Brisson Alain,
Ruiters Marcel H. J.,
Hoekstra Dick,
Engberts Jan B. F. N.
Publication year - 2000
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/(sici)1099-0690(200002)2000:4<665::aid-ejoc665>3.0.co;2-a
Subject(s) - pyridinium , amphiphile , chemistry , isothermal microcalorimetry , dna , transfection , zeta potential , pyridinium compounds , biophysics , organic chemistry , stereochemistry , nanotechnology , biochemistry , gene , copolymer , nanoparticle , polymer , physics , materials science , quantum mechanics , biology , enthalpy
Pyridinium amphiphiles have found practical use for the delivery of DNA into cells. Starting from 4‐methylpyridine, a general synthesis has been devised for the production of pyridinium amphiphiles which allows variation in both the hydrophobic part and in the headgroup area of the compounds. By means of differential scanning microcalorimetry, zeta potential, particle size measurements and cryo electron microscopy, some characteristics of the pyridinium amphiphile/DNA complexes have been determined.