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Enzymatic Synthesis of Galactose‐Containing Disaccharides Employing β‐Galactosidase from Bacillus circulans
Author(s) -
Farkas Erzsébet,
Thiem Joachim
Publication year - 1999
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/(sici)1099-0690(199911)1999:11<3073::aid-ejoc3073>3.0.co;2-5
Subject(s) - bacillus circulans , chemistry , trisaccharide , galactose , glycoconjugate , disaccharide , oligosaccharide , stereochemistry , enzyme , yield (engineering) , biochemistry , lactose , materials science , metallurgy
Galβ1→4 disaccharide structures are vital core units of the oligosaccharide components of glycoconjugates. β‐Galactosidase from Bacillus circulans (E.C.3.2.1.23) catalyses the transfer of galactose from a donor structure such as GalβO p NP to various GlcNAc and galactose derivatives, forming β1→4 linkages. The synthesis of several biologically relevant disaccharides {Galβ1→4GlcNAcαOAll ( 3 ), Galβ1→4GlcNAcβOAll ( 5 ), Galβ1→4GalαOAll ( 10 ), Galβ1→4GalβOAll ( 12 ), Galβ1→4GalβSPh ( 14 ), Galβ1→4GalβO p NP ( 16 ) and the trisaccharide Galβ1→4Galβ1→4GalβO p NP ( 18 )} has been achieved in 30–66 % yield by application of this enzyme.