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A Novel Sensitive Colorimetric Assay for Visual Detection of Solid‐Phase Bound Amines
Author(s) -
Madder Annemieke,
Farcy Nadia,
Hosten Noel G. C.,
De Muynck Hilde,
De Clercq Pierre J.,
Barry John,
Davis Anthony P.
Publication year - 1999
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/(sici)1099-0690(199911)1999:11<2787::aid-ejoc2787>3.0.co;2-d
Subject(s) - chemistry , combinatorial chemistry , solid phase synthesis , colorimetry , chromatography , phase (matter) , organic chemistry , biochemistry , peptide
A new simple and efficient method for the detection of incomplete coupling reactions during solid‐phase peptide synthesis is decribed. Using p ‐nitrophenyl ester 1 (NF31), free amino groups can be visually detected on the resin by direct coloring of the beads. A specific feature of the assay resides in the possibility of detection of sterically hindered primary amines.