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A Blue Excitable Charge‐Transfer Fluorescent Probe and Its Fluorogenic Derivative
Author(s) -
Goes Marijn,
Lauteslager Xavier Y.,
Verhoeven Jan W.,
Hofstraat Johannes W.
Publication year - 1998
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/(sici)1099-0690(199811)1998:11<2373::aid-ejoc2373>3.0.co;2-u
Subject(s) - chemistry , maleimide , fluorescence , photochemistry , adduct , acceptor , reagent , absorption (acoustics) , surface modification , molecule , derivative (finance) , organic chemistry , physics , quantum mechanics , acoustics , condensed matter physics , financial economics , economics
Structural modification of the highly fluorescent donor‐bridge‐acceptor molecule “Fluoroprobe” (FP) is shown to extend the excitation window to longer wavelengths. The resulting “Fluorotrope” (FT) shows appreciable absorption in the 350‐420‐nm range, so that visible (blue) light can be used for excitation. Further functionalization with a maleimide group results in the novel fluorogenic reagent MaleimidoFluorotrope (MFT) which yields fluorescent adducts with amines, thiols and other reactive groups that add to the double bond of the maleimide. The fluorescence wavelength of these adducts is extremely sensitive to the polarity and mobility of the medium.