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A Facile Solid‐Phase Synthesis of Oligonucleotides Containing a 3′−3′ Phosphodiester Bond for Alternate Strand Triple‐Helix Formation
Author(s) -
De Napoli Lorenzo,
Di Fabio Giovanni,
Messere Anna,
Montesarchio Daniela,
Piccialli Gennaro,
Varra Michela
Publication year - 1998
Publication title -
european journal of organic chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 1434-193X
DOI - 10.1002/(sici)1099-0690(199810)1998:10<2119::aid-ejoc2119>3.0.co;2-k
Subject(s) - phosphodiester bond , triple helix , chemistry , oligonucleotide , solid phase synthesis , stereochemistry , helix (gastropod) , combinatorial chemistry , dna , crystallography , biochemistry , rna , ecology , peptide , biology , snail , gene
An improved protocol for the synthesis of oligonucleotides containing a 3′−3′ phosphodiester linkage, for use in an alternate strand triple‐helix formation approach, is reported. Three 16‐mers and one 24‐mer have been efficiently synthesized and the corresponding triplexes have been analyzed by thermal denaturation experiments and CD spectroscopy.