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The turnover of phospholipid fatty acyl chains is activated by the insecticide Dieldrin in Bufo arenarum oocytes
Author(s) -
Fonovich de Schroeder Teresa,
Pechen de D’Angelo Ana M.
Publication year - 2000
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/(sici)1099-0461(2000)14:2<82::aid-jbt3>3.0.co;2-0
Subject(s) - dieldrin , phospholipid , chemistry , metabolism , glycerol , human fertilization , biochemistry , acylation , pesticide , biology , ecology , membrane , anatomy , catalysis
Dieldrin is a widespread environmental contaminant hazardous to many wildlife species. Some evidence obtained with Bufo arenarum oocytes indicates that Dieldrin decreases the fertilization rate in amphibian oocytes, but little is known about mechanisms by which the pesticide affects fertilization. Therefore, we investigated the effect of Dieldrin on oocyte phospholipid metabolism. Freshly obtained oocytes, prelabeled with 2 3 H‐glycerol or 9‐10 3 H palmitate, were exposed to 4 mg/L Dieldrin for 2 hours. Dieldrin reduced the amount of 2 3 H‐glycerol incorporation in all phosphoglycerides classes: PI, PA, PS, and SPH were affected in 80% of the cases and PC and PE were only reduced in 39% of the cases. The incorporation in neutral lipids was not affected. On the contrary, 9,10 3 H–palmitate incorporation increased in PC, PI, and PA, but TAG and FFA decreased. The more efficient incorporation of 3 H‐palmitate compared with 3 H‐glycerol in Dieldrin‐treated oocytes suggests the operation of an alternative route other than de novo synthesis for phospholipids. The retailoring of phosphoglycerides via a deacylation‐acylation pathway was demonstrated. These changes in phospholipid metabolism could be associated with the activation of certain enzymes produced by the pesticide. © 2000 John Wiley & Sons, Inc. J Biochem Toxicol 14: 82–87, 2000