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Citrinin‐induced mitochondrial permeability transition
Author(s) -
Da Lozzo Eneida J.,
Oliveira Maria Benigna M.,
Carnieri Eva Gunilla S.
Publication year - 1998
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/(sici)1099-0461(1998)12:5<291::aid-jbt5>3.0.co;2-g
Subject(s) - citrinin , dithiothreitol , chemistry , ruthenium red , membrane permeability , mitochondrion , mitochondrial permeability transition pore , efflux , biophysics , biochemistry , inner mitochondrial membrane , mycotoxin , membrane , calcium , biology , enzyme , apoptosis , food science , organic chemistry , programmed cell death
The effects of mycotoxin citrinin on Ca 2+ efflux and membrane permeabilization were studied in isolated rat liver mitochondria. The efflux rate observed when in presence of ruthenium red was higher when citrinin was added. Swelling experiments demonstrated Ca 2+ ‐dependent membrane permeabilization by citrinin. Catalase, butylhydroxitoluene (BHT), and dithiothreitol (DTT) did not protect swelling caused by Ca 2+ plus citrinin. The protection conferred by ATP–Mg 2+ and cyclosporin A in the latter experiments are strong indications of pore formation. These results suggest that citrinin can induce permeability transition by a mechanism that does not involve oxidative damage. © 1998 John Wiley & Sons, Inc. J Biochem Toxicol 12: 291–297, 1998