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Immediate‐Early Gene Expression during Regenerative and Mitogen‐Induced Liver Growth in the Rat
Author(s) -
Holden P. R.,
Odum J.,
Soames A. R.,
Foster J. R.,
Elcombe C. R.,
Tugwood J. D.
Publication year - 1998
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/(sici)1099-0461(1998)12:2<79::aid-jbt2>3.0.co;2-l
Subject(s) - microbiology and biotechnology , gene expression , mitogen activated protein kinase , liver regeneration , gene , chemistry , biology , regeneration (biology) , genetics , signal transduction
The nongenotoxic carcinogens phenobarbitone (PB) and methyl clofenapate (MCP) and the hepatomitogen pregnenolone 16α carbonitrile (PCN) are direct inducers of hepatic S ‐phase in rats, whereas the S ‐phase seen after partial hepatectomy is regenerative. We have investigated S ‐phase and immediate‐early gene expression (c‐ myc and c‐ jun ) in rat liver following these treatments to study the differences in gene expression associated with direct vs. regenerative responses. Both partial hepatectomy (one‐ and two‐thirds) and mitogen treatment caused an increase in hepatic S ‐phase that peaked around 36 hours. Two‐thirds partial hepatectomy caused the greatest increase in S ‐phase followed by one‐third partial hepatectomy, then the mitogens PCN, MCP, and PB in that order. This order of response was also seen with c‐ jun and to a lesser degree with c‐ myc expression, suggesting that immediate‐early gene expression might be linked not only to regenerative S ‐phase but also to direct mitogen‐induced responses. © 1997 John Wiley & Sons, Inc. J Biochem Toxicol 12: 79–82, 1998