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Regulatory DNA elements of phenobarbital‐responsive cytochrome P450 CYP2B genes
Author(s) -
Honkakoski Paavo,
Negishi Masahiko
Publication year - 1998
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/(sici)1099-0461(1998)12:1<3::aid-jbt2>3.0.co;2-p
Subject(s) - enhancer , promoter , transcription (linguistics) , transcription factor , response element , phenobarbital , gene , dna , biology , microbiology and biotechnology , genetics , gene expression , pharmacology , linguistics , philosophy
This article reviews recent progress in characterizing cis ‐acting DNA elements of the phenobarbital‐inducible CYP2B genes. Whereas proximal DNA elements such as the C/EBP binding site regulate basal transcription activity, phenobarbital‐responsive enhancer activity is governed by the distal element (designated phenobarbital‐responsive enhancer module, PBREM) residing about ‐2.3 kbp upstream from the transcription start site. Proximal elements are not required to enhance the phenobarbital‐inducible transcription, since the PBREM can confer the inducibility to several heterologous promoters. Repression of the basal transcription by a negative element upstream of the ‐0.8 kbp region, however, may be necessary for the proper regulation of the CYP2B genes. © 1997 John Wiley & Sons, Inc. J Biochem Toxicol 12: 3–9, 1998