Open AccessRapid colorimetric assay of urinary β‐galactosidase and N ‐acetyl‐β‐glucosaminidase with cobas mire auto‐analyzer
Author(s) -
Xu Guobing,
Zhu Lihua,
Hong Jianmei,
Cao Yi,
Xia Tiean
Publication year - 1999
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1999)13:3<95::aid-jcla1>3.0.co;2-l
Subject(s) - chromatography , chemistry , substrate (aquarium) , urine , incubation , ionic strength , urinary system , enzyme , mire , biochemistry , medicine , biology , organic chemistry , ecology , aqueous solution , peat
A rapid and simple colorimetric method for the assay of urinary β‐galactosidase (GAL) and N ‐acetyl‐β‐ D ‐glucosaminidase (NAG) using 4‐nitrophenyl‐glycosides as the substrates and a Cobas Mire Auto‐analyzer is described. Optimal conditions for this method including substrate concentration, ionic strength, pH and incubation time were characterized. Endogenous substances in urine did not interfere with the assays. Incubation time could be shortened to 10 minutes. A small volume of an alkaline buffer to terminate the enzyme reaction achieved better sensitivity and accuracy. Buffer‐substrate solutions were stable for at least one week at 4°C. Normal values of the two urinary enzymes are also reported. J. Clin. Lab. Anal. 13:95–98, 1999. © 1999 Wiley‐Liss, Inc.