Open AccessImmunoturbidimetric assay of glycated hemoglobin
Author(s) -
Metus Paolo,
Ruzzante Nicoletta,
Bonvicini Piero,
Meneghetti Martina,
Zaninotto Martina,
Plebani Mario
Publication year - 1999
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1999)13:1<5::aid-jcla2>3.0.co;2-s
Subject(s) - glycated hemoglobin , hemoglobin , chemistry , medicine , diabetes mellitus , biochemistry , endocrinology , type 2 diabetes
We investigated the performances of HbA1c determination by a latex enhanced turbidimetric immunoassay using the specific monoclonal antibodies (Unimate, Roche) against the β‐N‐terminal fragments. The coefficients of variation ranges from 1.7 to 3.8% within assay (n = 30) and from 3.9 to 4.9% between assay (n = 20). The assay was linear from 2.5 to 14.9% of HbA1c. No interferences was found from fetal, carbamylated, or variant (S) hemoglobins and from labile Schiff adduct with glucose. The following relationship was derived from fresh sample comparison between HPLC (Diamat‐BioRad) ( x ) and immunoassay ( y ) method: y = 0.971 x + 0.87%, r = 0.98, n = 115. The immunoassay provides a highly precise and specific method for HbA1c. J. Clin. Lab. Anal. 13:5–8, 1999. © 1999 Wiley‐Liss, Inc.