Open AccessTwo color analysis of HLA‐B27 antigen by flow cytometer—A comparative study by conventional microlymphocytoxicity, DNA genotyping polymerase chain reaction and flow cytometric measurement
Author(s) -
Chou SuJen,
Lai NingSheng,
Su JenPey,
Wu JiaLin,
Lan JoungLiang
Publication year - 1997
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1997)11:6<369::aid-jcla11>3.0.co;2-u
Subject(s) - hla b27 , genotyping , polymerase chain reaction , antigen , microbiology and biotechnology , ankylosing spondylitis , serology , dna , human leukocyte antigen , antibody , medicine , immunology , biology , genotype , genetics , gene
For evaluation of specificity and sensitivity of flowcytometric determination of HLA‐B27 antigen, we determined the HLA‐B27 on lymphocytes using HLA‐B27 monoclonal antibody by flow cytometer. Data were compared to those by conventional Terasaki microlymphocytoxicity test and DNA genotyping Polymerase Chain Reaction (PCR) method. One hundred and ninety four patients with various forms of arthritis were included in this study. Forty one of them were HLA‐B27 positive, confirmed by three methods concomitantly with complete accordance. None of serological B27 negative, B7 CREG positive cells were found to be flowcytometric fluorescence positive. Furthermore, there was no significant difference of B27 intensity between different B27 DNA subtypes, nor was there any difference between primary ankylosing spondylitis (AS) and other secondary spondylitis patients as measured by mean channel of fluorescence. It is suggested that flowcytometric measurement of HLA‐B27 antigen is a rapid and reliable method for HLA‐B27 determination. J. Clin. Lab. Anal. 11:369–373, 1997. © 1997 Wiley‐Liss, Inc.