Open AccessFast isolation of RNA to detect expression of tumor markers
Author(s) -
Schenk Jörg A.,
Hillebrand Timo,
Lübbe Lieselotte,
Heymann Stephan,
Böttger Michael,
Micheel Burkhard,
Bendzko Peter
Publication year - 1997
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1997)11:6<340::aid-jcla5>3.0.co;2-9
Subject(s) - isolation (microbiology) , expression (computer science) , rna , biology , rna extraction , computational biology , microbiology and biotechnology , genetics , computer science , bioinformatics , gene , programming language
The expression status of several tumor‐related proteins is of great interest in clinical examination and research. As a completion to conventional antibody staining, RT‐PCR is often used today. Reliable isolation of RNA from a low number of cells is very often a critical stage of such an examination. We demonstrate here a simple and fast method to isolate RNA from only 10,000 cells and applied it to the detection of CEA, c‐ERB‐B2, and mdr‐1 as often studied models for tumor markers. J. Clin. Lab. Anal. 11:340–342, 1997. © 1997 Wiley‐Liss, Inc.