Open AccessEvaluation of recent techniques for detection of red blood cell antibodies in sera of reference samples, patients, pregnant women, and blood donors
Author(s) -
de Castilho Lilian M.,
Pellegrino Jordão,
Bechelli Ana Paula P.,
Le Pennec Pierre Yves,
Mendes Nelson F.
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:5<250::aid-jcla4>3.0.co;2-c
Subject(s) - antibody , peg ratio , polyethylene glycol , ionic strength , chemistry , papain , chromatography , microbiology and biotechnology , immunology , medicine , biology , biochemistry , enzyme , finance , aqueous solution , economics
The sensitivity of the low ionic strength solution antiglobulin test (LISS‐AGT), polyethylene glycol antiglobulin test (PEG‐AGT), low ionic strength solution solid‐phase anti‐globulin test (LISS‐SPAT), gel low ionic strength solution antiglobulin test (GEL‐LISS), and gel papain test (GEL‐PAP) was compared in titration studies of 460 sera containing identified IgG alloantibodies. The GEL‐PAP was 100% sensitive to detect Rh antibodies, whereas the PEG‐AGT was the most sensitive to detect Kell, Duffy, Kidd, Ss, and rare blood group antibodies. The better performance of PEG‐AGT was especially obvious with Kell, Duffy, and Ss antibodies (S = 100%). When the sensitivity of the LISS‐AGT, PEG‐AGT, GEL‐LISS, and GEL‐PAP was evaluated in different routines, the GEL‐LISS showed to be more sensitive than PEG‐AGT in the detection of clinically significant antibodies. These discrepant results showed that the performance of a technique may change when it is applied as a routine. © 1996 Wiley‐Liss, Inc.