Open AccessOptimal conditions for collection of blood samples for assay of α 2 ‐macroglobulin trypsin complex‐like substance (MTLS)
Author(s) -
Kato Masanori,
Tatemichi Noriyuki,
Hayakawa Shinobu,
Naruse Satoru,
Kitagawa Motoji,
Nakae Yasuyuki,
Hayakawa Tetsuo
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:5<239::aid-jcla2>3.0.co;2-3
Subject(s) - blood collection , chemistry , heparin sodium , chromatography , blood sampling , sodium citrate , trypsin , heparin , medicine , biochemistry , enzyme , pathology , emergency medicine
We have developed an enzyme‐linked immunosorbent assay (ELISA) for the specific quantification of α2‐macroglobulin‐trypsin complex‐like substance (MTLS). To exclude artifacts in measured values of MTLS, the conditions for collection of blood samples are critical. In the present study, we have determined the optimal conditions for blood collection and investigated the role of MTLS as a clinical tool for diagnosis in pancreatitis. Results obtained are as follows: (1) MTLS levels of all sera were more than 10‐fold higher than the corresponding plasma; (2) MTLS levels of heparinized plasma were the lowest among plasma with three anticoagulants (sodium citrate, sodium EDTA and heparin); (3) some kinds of blood collection tubes containing heparin were not suitable for the sampling; (4) MTLS values of plasma obtained by blood collection tubes containing Trasylol® and sodium EDTA were demonstrated more stable and lower than those obtained by heparin tubes; and (5) under these conditions, we can exclude elevation of MTLS values caused by inappropriate blood sampling and find the time course of the elevation reflecting clinical course of a patient with acute pancreatitis and a patient after endoscopic retrograde cholangiopan‐creatography (ERCP). The optimal conditions for collection of blood samples were as follows. Blood sampling should be performed by blood collection tubes containing Trasylol® (50 μl/ml blood) and sodium EDTA (1.5 mg/ml blood). The samples were immediately stored at 4°C and centrifuged at 3,000 rpm for 15 min. The plasma were stored in plastic tubes at 4°C until assayed. © 1996 Wiley‐Liss, Inc.