Open AccessTechnicon Immuno 1® PSA assay measures both free and alpha‐1‐antichymotrypsin‐complexed prostate‐specific antigen on an equimolar basis
Author(s) -
Zhou Zeqi,
Ng Phillip C.,
Very Donald L.,
Allard W. Jeffrey,
Yeung Kwok K.
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:3<155::aid-jcla8>3.0.co;2-a
Subject(s) - autoanalyzer , prostate specific antigen , immunoassay , chemistry , chromatography , polyclonal antibodies , antigen , prostate cancer , urology , microbiology and biotechnology , medicine , cancer , antibody , immunology , biology
In this study, we investigated the immunoreactivity of the Technicon Immuno 1® PSA assay, a monoclonal‐polyclonal sandwich immunoassay, with free and ACT‐complexed PSA. Assay calibrators prepared with free PSA (standard immuno 1 calibrators) and calibrators consisting of 90% ACT‐complexed and 10% free PSA (90:10 calibrators) yielded virtually identical PSA recoveries at all concentrations tested. Concentrations of total PSA at ∼4 and 10 ng/mL, prepared in varying ratios of free PSA to PSA‐ACT complex, recovered from 92–104% at 4 ng/mL and from 98–102% at 10 ng/mL. Additionally, an excellent correlation of serum total PSA values from a panel of 40 prostatic cancer patient samples was obtained using calibration curves generated with the standard Immuno 1 calibrators or the 90:10 calibrators. These results demonstrate that the Technicon Immuno 1® PSA assay measures free and ACT‐complexed PSA on an equal molar basis. © 1996 Wiley‐Liss, Inc.