Open AccessA new, fast, and simple DNA extraction method for HLA and VNTR genotyping by PCR amplification
Author(s) -
Planelles D.,
Llopis F.,
Puig N.,
Montoro J. A.
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:3<125::aid-jcla2>3.0.co;2-a
Subject(s) - genotyping , dna extraction , human leukocyte antigen , polymerase chain reaction , simple (philosophy) , dna , biology , genetics , computational biology , genotype , gene , antigen , philosophy , epistemology
In the present study a new DNA extraction method is described. The new protocol, which uses caprylic acid for isolating DNA, is technically simple and very fast, as it enables us to obtain DNA from peripheral blood in only 10 minutes. Moreover, DNA preparations obtained with this procedure can be effectively used for HLA class II and variable number tandem repeat genotyping by polymerase chain reaction, so the new method is well suited for routine clinical use in any type of analysis requiring DNA typing for individual characterization. © 1996 Wiley‐Liss, Inc.