Open AccessMeasurement of soluble thrombomodulin in sera from various clinical stages of diabetic nephropathy
Author(s) -
Rinno Hisaki,
Kuramoto Takao,
Iijima Toshihiko,
Yagame Mitsunori,
Tomino Yasuhiko
Publication year - 1996
Publication title -
journal of clinical laboratory analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 50
eISSN - 1098-2825
pISSN - 0887-8013
DOI - 10.1002/(sici)1098-2825(1996)10:3<119::aid-jcla1>3.0.co;2-6
Subject(s) - medicine , diabetic nephropathy , diabetes mellitus , nephropathy , creatinine , thrombomodulin , endocrinology , albuminuria , albumin , microalbuminuria , gastroenterology , platelet , thrombin
The levels of soluble thrombomodulin (TM) in serum samples were measured by one‐step sandwich enzyme immunoassay. The aim of the present study was to determine if levels of soluble TM in sera might correlate with disease activity in patients with diabetic nephropathy. Three hundred and twenty patients with diabetic nephropathy were examined. Patients with diabetic retinopathy were excluded from the present study. This study showed an increase of soluble TM levels in sera from patients with diabetic nephropathy. The levels of soluble TM in sera from the macroalbuminuric stage with renal dysfunction were significantly increased compared with those from the normo‐, micro‐, or macroalbuminuric stage of diabetic nephropathy without renal dysfunction. The increase of soluble TM in sera paralleled levels of urinary albumin, blood urea nitrogen (BUN), s‐creatinine (Cr), and duration of noninsulin‐dependent diabetes mellitus (NIDDM). Furthermore, a decrease of TM staining in the glomerular capillary walls was observed in both microalbuminuric and macroalbuminuric stages by immunofluorescence. It appears that the measurement of soluble TM in sera is useful in evaluating the degree of glomerular endothelial injuries in patients with diabetic nephropathy. © 1996 Wiley‐Liss, Inc.