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TransgenICSI reviewed: Foreign DNA transmission by intracytoplasmic sperm injection in rhesus monkey
Author(s) -
Chan Anthony W.S.,
Luetjens C. Marc,
Dominko Tanja,
RamalhoSantos Joǎo,
Simerly Calvin R.,
Hewitson Laura,
Schatten Gerald
Publication year - 2000
Publication title -
molecular reproduction and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 105
eISSN - 1098-2795
pISSN - 1040-452X
DOI - 10.1002/(sici)1098-2795(200006)56:2+<325::aid-mrd25>3.0.co;2-n
Subject(s) - biology , intracytoplasmic sperm injection , andrology , embryo , sperm , blastomere , blastocyst , human fertilization , oocyte , embryogenesis , fertilisation , in vitro fertilisation , genetics , reproductive technology , medicine
This brief review considers the status of transgenesis by intracytoplasmic sperm injection (ICSI) with nonhuman primates. GFP expressing rhesus macaques embryos (mean = 34.6%; N = 81) were produced by ICSI using rhodamine‐tagged DNA encoding the green fluorescence protein (GFP) gene bound on sperm. Rhodamine signal was lost at the egg surface during in vitro fertilization (IVF) but could be traced by dynamic imaging during ICSI within the egg cytoplasm. GFP gene was expressed as early as the 4‐cell stage in ICSI embryos but not in embryos produced by in vitro fertilization (IVF). The percentage of GFP expressing blastomeres increased during embryogenesis to the blastocyst stage. Three offspring resulted from seven embryo transfers—a set of anatomically normal twins (a male and a female) stillborn 35 days premature, and a healthy male born at term. Although transgene was not detected in the offspring, the successful production of live primates using DNA bound sperm by ICSI suggests an alternative route to creating transgenic animals. It also raises concern regarding transmission of infectious material during ICSI. Mol. Reprod. Dev. 56:325–328, 2000. © 2000 Wiley‐Liss, Inc.